ANTI-INFLAMMATORY STUDY (IN-VITRO AND IN-VIVO) AND HPTLC DENSITOMERIC DETERMINATION OF QUERCETIN FROM METHANOLIC LEAF EXTRACT OF HOTTUYNIA CORDATA THUNB. FROM MIZORAM, INDIA

Objective: This study aimed to investigate the anti-inflammatory activity of methanolic leaf extract Houttuynia cordata Thunb. from Mizoram, India, using in-vitro and in-vivo models. In addition, also determine quercetin content high-performance thin-layer chromatography (HPTLC) densitometry. Methods: The H. was prepared successive Soxhlet extraction, its evaluated models such as inhibition protein denaturation proteinase activity. carrageenan-induced paw edema in rats. determined HPTLC Results: showed significant both exhibited a dose-dependent significantly reduced rat model. IC50 values egg albumin bovine serum studies were 317.365 μg/mL 198.918 μg/mL, respectively. results that plant 200 mg/kg b.w. 400 b.w., which considerably edema, their effects comparable those standard diclofenac sodium. densitometry analysis revealed presence extract, with 21.8 ng/μL. Conclusion: India demonstrated may contribute properties. These findings provide scientific evidence for traditional use an agent.